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Table 4 Effect of ternary combinations of natural extracts on yeast in the presence of control conditions (YPD without stress), saline (NaCl and LiCl), osmotic (Sorbitol) and temperature (10 °C and 37 °C) stress

From: A fast method to evaluate in a combinatorial manner the synergistic effect of different biostimulants for promoting growth or tolerance against abiotic stress

 

No-stress

NaCl 1 M

LiCl 0.15 M

Sorbitol 1.8 M

37 °C

10 °C

B2–B3

=

+

+

=

=

=

B1–B2–B3

=

+

+

+

=

=

B2–B3–B4

=

+

+

++

=

=

B2–B3–B5

=

+

+

+

=

=

B2–B3–B6

=

+

+

+

=

=

B2–B3–B7

=

+

+

+

=

=

B2–B3–B8

=

=

B2–B3–B9

=

B2–B3–B10

=

+

+

=

B2–B5

=

+

+

++

=

+

B1–B2–B5

=

+

+

+

B2–B3–B5

=

+

+

+

W

=

B2–B4–B5

=

+

=

+

=

B2–B5–B6

=

=

=

+

=

=

B2–B5–B7

=

+

+

+

=

B2–B5–B8

=

=

=

=

B2–B5–B9

B2–B5–B10

=

+

=

=

W

=

B3–B4

=

+

+

++

=

=

B1–B3–B4

=

+

+

++

=

=

B2–B3–B4

=

+

+

++

=

=

B3–B4–B5

=

+

+

+

=

=

B3–B4–B6

=

+

=

++

=

=

B3–B4–B7

=

+

+

++

=

=

B3–B4–B8

=

=

B3–B4–B9

=

B3–B4–B10

=

+

=

+

=

=

  1. In ternary combinations, S. cerevisiae was grown in a fresh YPD medium containing 400 µg/ml of each product, except for B8 and B9, which used 100 µg/ml. Three independent complete experiments, each using three biological and three technical replicates per plate, were performed with similar results
  2. (++) High growth stimulation; (+) Growth stimulation; (=) no observable effect; (−) negative; (W) weak