Fig. 4
From: DeLTa-Seq: direct-lysate targeted RNA-Seq from crude tissue lysate
Successful application of DeLTa-Seq with A. thaliana seedlings. a A schematic diagram of DeLTa-Seq library preparation. P7 and P5 are adapter sequences binding with the oligonucleotides on the flow cells supplied by Illumina. Adp 1 and Adp 2 are adapter sequences for the sequencing. UMI indicates unique molecular identifier. b Scatter plot of log2 RPM + 1 values of each gene in targeted RNA-Seq and non-targeted RNA-Seq of lysate of A. thaliana seedlings. c The bar graph represents the ratio of reads corresponding to target and non-target genes in targeted RNA-Seq and non-targeted RNA-Seq