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Table 1 The rhPCR primer sequences designed to cluster Plasmodiophora brassicae pathotype DNA

From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters

Primer pair

Primer name

Primer sequence (5′-3′)c

rh1-43812Ra

rh1-43812Rfw

ACGACGACCCGGACACCATCGCrUAACGC/3SpC3/

rh1-43812Rrv

TTGGCGATGGGCGCCACCrUGCGAT/3SpC3/

rh1-43812Ab

rh1-43812Afw

GCGACGTCCCGGACACTATCGTrCAACGC/3SpC3/

rh1-43812Arv

TTGGCGATGGTCGCCACCrGGCGAT/3SpC3/

  1. aPrimer pair to amplify pathotypes of the reference cluster (pathotype 5X)
  2. bPrimer pair to amplify pathotypes of the alternate cluster (pathotype 3H)
  3. cThe discriminatory SNPs are represented by the bolded alleles. The ribonucleotide residue is preceded by a lower case 'r', followed by the mismatched nucleotide five bases downstream; the C3 spacer is indicated at the 3’ end of the primers
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Plant Methods

ISSN: 1746-4811