Fig. 7
From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters
Results of a single-blind evaluation of Plasmodiophora brassicae field and single-spore isolates with the rhPCR assay. Amplification products were resolved by electrophoresis on a 1% (w/v) agarose gel prepared with Tris–acetate-EDTA buffer and SYBR Safe gel stain. A GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific, Waltham, MA, USA) was included in the leftmost lane as the marker. The primer pairs rh1-43812R (upper panel) and rh1-43812A (bottom panel) were used to cluster the 16 blinded galls. Samples were disclosed to be isolates SACAN-ss3, ORCA-ss4, AbotJE-ss1, CDCN-ss3, ST27, ST40, LG-2, F3-14, F1-14, CDCN #4-14, SW46, F185-14, SS23, LG-3, SACAN-03-1, and SC14 (lanes 1–16). The negative control is displayed in the rightmost lane, as represented by the N