Fig. 1
From: Protocol: rhPCR and SNaPshot assays to distinguish Plasmodiophora brassicae pathotype clusters
Testing of the specificity of the rhPCR assay with gBlocks. Amplicons were resolved by electrophoresis on 1% (w/v) agarose gels prepared with Tris-acetate-EDTA buffer and SYBR Safe gel stain. A GeneRuler 1 kb Plus DNA Ladder (Thermo Scientific, Waltham, MA, USA) was included in the leftmost lane as the marker. The primer pairs rh1-43812R (upper panel) and rh1-43812A (bottom panel) were evaluated against the gBlocks. Two replicates of the reference 5X cluster gBlock and two replicates of the alternate 3H cluster gBlock were ran with each primer pair. The negative control is displayed in the rightmost lane, as represented by the N