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Fig. 4 | Plant Methods

Fig. 4

From: Generation of the salicylic acid deficient Arabidopsis via a synthetic salicylic acid hydroxylase expression cassette

Fig. 4

Pathogen resistance of the S5Hpro::EGFP-S3H transgenic plants to Pst DC3000. a The disease symptoms of WT, NahG, single-copy S5Hpro::EGFP-S3H transgenic plants at 1 and 3 days after Pst DC3000 infection. b Quantification of the growth of Pst DC3000 in plants of a at 0, 1, 3 days post inoculation (DPI). The data are means ± SE (n = 6 biological replications). The levels of free SA (c) and total SA (d) in WT, NahG and S5Hpro::EGFP-S3H transgenic plants after Pst DC3000 infection. The data are means ± SE (n = 4 biological replications). Quantification of the PR1 (e) and PR2 (f) expression in WT, NahG and S5Hpro::EGFP-S3H transgenic plants after Pst DC3000 infection by qPCR. The data are means ± SE (n = 3 biological replications). Statistical differences among replicates are labeled with different letters (P < 0.05, one-way ANOVA and post-hoc Tukey’s test)

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Plant Methods

ISSN: 1746-4811