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Fig. 1 | Plant Methods

Fig. 1

From: Generation of the salicylic acid deficient Arabidopsis via a synthetic salicylic acid hydroxylase expression cassette

Fig. 1

Design of a synthetic SA hydroxylase expression cassette catalyzing SA to 2,3-DHBA. a Vector map for S3H gene overexpression under S5H promoter. The S5H promoter of Arabidopsis was cloned and constructed into the plant expression vector (pPZP-RCS2) to drive the expression of EGFP-S3H. The map was prepared by SnapGene. b The principle of a feedback loop for SA catabolism to 2,3-DHBA in the S5Hpro::EGFP-S3H transgenic plants. The S5H promoter can be induced by SA and then drive the expression of EGFP-S3H. The expressed S3H enzyme can convert SA into 2,3-DHBA and reduce the SA levels

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Plant Methods

ISSN: 1746-4811