Fig. 5

Tethering of a ribosomal protein translation factor to the SOC1 mRNA boosts protein production. A Translational fusion between FLAG-BD and the RPS6 ribosomal protein generates the BD + R protein. B Flowering time of T2 BD + R plants grown side-by-side with wt Col. + T plants inherited the BD + R transgene, -T plants are siblings that did not inherit the transgene. C Representative images of T2 BD + R (+ T) plants grown side-by-side with wt Col taken 24 days after germination. D Western blot of SOC1 protein accumulation in seven biological replicates of early-flowering plants of the BD + R line compared to wt Col. PEP is an unrelated protein used as a loading control. E Quantification of SOC1 protein accumulation in the BD + R line from part (D). Individual biological replicates are shown as blue points. Bar height, error bars and statistics are the same as Fig. 2B. F qRT-PCR of SOC1 polyadenylated mRNA. Three biological replicates of each genotype are shown as red points. Bar height, error bars and statistics are the same as Fig. 2B. G qRT-PCR of SOC1 nascent transcripts (unspliced and not polyadenylated). Three biological replicates of each genotype are shown as red points. Bar height, error bars and statistics are the same as Fig. 2B