Skip to main content
Fig. 5 | Plant Methods

Fig. 5

From: MoBiFC: development of a modular bimolecular fluorescence complementation toolkit for the analysis of chloroplast protein–protein interactions

Fig. 5

OEP7-mTRQ is a suitable reference FP for ratiometric chloroplastic BiFC. A Outline of multigenic BiFC construct encoding an OEP7-mTRQ reference FP that was used for B BiFC assays between two protein pairs in N. benthamiana mesophyll cells. Scale, 10 µm; histogram levels shown at the bottom of each channel. C Normalised BiFC signals were calculated as the ratio between total YFP and TRQ fluorescence. The negative control (HSP21-cYFP/mCHERRY-nYFP) was set to 1. Bar indicates mean and cross indicates median ± 95% confidence interval (n = 100 transformed cells). Significance was calculated using the Kruskal Wallis test, groups are indicated by lower case letters (P < 0.001). n, nYFP; c, cYFP. Modules and plasmids are described in Additional file 2: Table S1 and full plasmid sequences are available in Additional file 4

Back to article page

Plant Methods

ISSN: 1746-4811