Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR

Table 4 CGMMV detection by four methods in low-concentration virus-infected seed

Infected: healthy seed mass ratio	Mass percentage (%) ^b	Subsamples^c (m)	Percentage of positive results^d (positive detection rate, % ^e)
Infected: healthy seed mass ratio	Mass percentage (%) ^b	Subsamples^c (m)	Method A	Method B	Method C	Method D
1:900	0.111%	20	20 (100%)	20 (100%)	20 (100%)	0 (0%)
1:1000	0.1%	20	20 (100%)	0 (0%)	2 (10%)	0 (0%)
LOD (mass percentage, %)^f			0.1	0.111	0.111	N.T. ^g

CGMMV Cucumber green mottle mosaic virus, LOD limit of detection
^aThe four methods: (A) one-step pre-amplification RT-qPCR; (B) universal RT-qPCR [14, 24]; (C) universal RT-qPCR for double-quenchen probes [4]; (D) DAS–ELISA
^bMass percentage of infected seeds in total seeds, %
^cnumber of subsamples, m = 20
^dNumber of positive results among 20 subsamples
^ePercentage of the number of positive results detected in the total number of subsamples, %
^fUnder the 95% confidence probability, the positive number of 20 sub samples is detected
^gN.T., not tested

ISSN: 1746-4811