Table 1 Primers and probes used for the detection of CGMMV in seeds
Primer | Sequence (5′-3′) | Position | Size of the PCR product (bp) | Reference |
|---|---|---|---|---|
Fw1 | ATCCGGAGTTTTCGATTA | 257–274 | 96 | This work |
Rev1 | GCATCATCATATATTCCAATTC | 331–352 | ||
Probe1 | FAM-TTACCGCCACCAAGAACTCTGT-BHQ1 | 278–299 | ||
Fw2 | GCATAGTGCTTTCCCGTTCAC | 6284–6304 | 101 | |
Rev2 | TGCAGAATTACTGCCCATAGAAAC | 6361–6384 | ||
Probe2 | FAM-CGGTTTGCTCATTGGTTTGCGGA-BHQ1 | 6315–6337 | ||
Fw3 | TTGGGCGTTGTGGTTTCTG | 5209–5227 | 90 | [4] |
Rev3 | AGAATGCATCCTTGTGTCAACC | 5277–5298 | ||
Probe3 | FAM-AATCCTGTAGGGGTGGTGCTACTGT-DQ1 | 5245–5270 |