Fig. 3
From: Determination of protoplast growth properties using quantitative single-cell tracking analysis
Selection of isolated single cells for tracking analysis. Bright field images of a time series of immobilized tobacco wild-type protoplasts, recorded at 1, 3 and 4 days after immobilization (DAI). A Protoplast growth is affected by contact with neighboring cells if positioned too close to each other (red arrow) while only single cells are able to expand isodiametrically (green arrow). B The property of segmented cell clusters to split into multiple objects after application of the watershed algorithm was exploited by adapting the Speckle Instructor plugin to filter for single cells exclusively