Fig. 1
From: Determination of protoplast growth properties using quantitative single-cell tracking analysis
AtBAG4 localization during proliferation. Nicotiana tobacum protoplasts expressing YFP-AtBAG4 were immobilized and recorded in bright field and epifluorescence modes for 13 days after immobilization (DAI). While YFP-AtBAG4 is almost exclusively nuclear localized immediately after isolation at DAI0, YFP-AtBAG4 is found also in cytoplasmic membranes with continued incubation until DAI3 and increases massively in microcalli after completion of cell divisions. Note also the increased fluorescence in contact areas of adjacent protoplasts. Bar = 50 µm