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Fig. 7 | Plant Methods

Fig. 7

From: Root-TRAPR: a modular plant growth device to visualize root development and monitor growth parameters, as applied to an elicitor response of Cannabis sativa

Fig. 7

Summarized workflow of plant growth experiment in this study. First, industrial hemp seeds were germinated in Petri dishes, while Root-TRAPR systems were assembled. Seedlings were transferred to Root-TRAPR systems on day 0. Chitosan treatment was performed on day 7. Plant tissues (shoot and root) and root exudate were sampled on day 14. Tissue samples were ground and divided into three parts for subsequent analyses. They were analyzed for bioassays (protein), phytohormone quantification (metabolite) and gene detection (DNA). Root exudate was passed through a 10 kDa MWCO Amicon centrifugal filter device (Merck Millipore, Germany) to concentrate proteins, then tested for enzyme activities. This figure was partially created with BioRender.com

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Plant Methods

ISSN: 1746-4811