A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants

Table 1 Different transfection reagents were assessed for transfection efficiency in protoplasts indicated by EGFP presence

Transfection reagent	Concentration^a	EGFP presence	Cell health
Chariot™	0.25 µl	Yes	+ ^b
Escort™	25 µl	No	+
FuGENE®	5.0 µl	No	+ +
Lipofectamine® 3000	0.5 µl	Yes	+ +
Lipofectamine® LTX with plus™ reagent	0.5 µl	Yes	+ + +
TransIT®-2020	1.0 µl	No	+ +
X-fect™	7.5 µl	No	+

^aRepresents the concentration of transfection reagents mixed with 25 µg of plasmid DNA/ 100 protoplast (1.5 × 106 cells per mL)
^bThe effects of the transfection reagents on protoplast cell health and division were assessed. Poor (less than 20% intact protoplasts; +), good (over 50% intact protoplasts; + +), excellent (over 90% intact protoplasts; + + +)

ISSN: 1746-4811