Fig. 4

Evaluation of OCS reporter gene expression in transgenic Arabidopsis plants. A Image showing Kanamycin selection of the transgenic Arabidopsis seedlings on MS media. Seedlings highlighted in the red circle have successfully incorporated OCS circuit. Transformation efficiency is within reasonable ranges (~ 1%) determined by a simple evaluation of the identified seedlings. B Fluorescence microscope image of Arabidopsis transgenic T₁ plants containing the constitutive expression of YFP under the OCS control (OCS 1–1). Scale bar: 50 μm C. Image showing Kanamycin selection of the transgenic Arabidopsis seedlings on MS media using luminescence reporter (OCS4-1) taken using the NightOwl (Methods). D. Image of a T₁ Arabidopsis plant containing OCS4-1 at the rosette stage after spraying the luciferin (Methods) containing OCS4-1. This image, taken at the rosette stage using NightOwl after luciferin spray, shows that the luciferase expression is active throughout the adult plant. A non-transgenic plant on the left was used as a negative control in the luminescence reporter assay