Fig. 1

Schematic overview of the design-build-test cycle A Genetic elements such as promoters, genes and terminators are encoded as modular parts consisting of BsaI recognition sites flanked by specific overhangs to ensure the hierarchical assembly of transcriptional units. Once assembled, the constructs are transformed into Agrobacterium and the reporter expression is characterized in Nicotiana benthamiana leaf infiltrates B Design of the shuttle vector backbone used for the assembly of constructs and subsequent propagation in Agrobacterium