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Fig. 7 | Plant Methods

Fig. 7

From: Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE

Fig. 7

Polypeptide patterns of PSI-LHCII supercomplexes separated from mesophyll thylakoids of lincomycin treated maize. a BN/SDS gel pattern of thylakoids. Points refer to PSII supercomplexes. Thylakoids (500 µg Chl mL−1) were solubilised using 1% (w/V) β-DM plus 1% (w/V) digitonin, and complexes were separated in 4.3–8% gel gradients followed by SDS PAGE. PSI-LHCII*: large PSI-LHCII band. b Comparison of the polypeptide profiles of PSI and PSI-LHCII supercomplexes normalised to PSI core 1–3 (see in a. Variations in the amounts of polypeptides in the different complexes were compared using two-way ANOVA with Tukey’s multiple comparison test [P < 0.05; n = 2 × 3 (biological × technical)]. Significant differences are indicated separately in each polypeptide/LHCII-t group by different letters

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