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Fig. 1 | Plant Methods

Fig. 1

From: Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE

Fig. 1

BN/SDS PAGE pattern of poplar (Populus jacquemontiana var. glauca) thylakoid complexes. a Thylakoids (500 µg Chl mL−1) were solubilised with 2% (w/V) β-DM and separated in 5–12% BN gel gradient followed by SDS PAGE. C, S and M: PSII core, strongly (S) and moderately bound (M) LHCII trimers; CP: chlorophyll-protein; LHCII-a: CP29 + CP24 + LHCII-t; s: supercomplex; t: trimer d: dimer; m: monomer; Cyt: cytochrome; ATPs: ATP synthase; CF1: coupling factor 1. Protein standards (kDa) were as follows: bovine serum albumin (66), ovalbumin (45), glyceraldehyde-3-phosphate dehydrogenase (36), carbonic anhydrase (29), trypsinogen (24), trypsin inhibitor (20), α-lactalbumin (14). b Baseline of the densitogram of poplar thylakoid complexes separated by BN PAGE shown in a made by the Phoretix program (adjusted by the user)

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