Table 2 Recovery percentages of amino acids after MCX-SPE cleaning
From: A glass bead semi-hydroponic system for intact maize root exudate analysis and phenotyping
a. Group 1 of amino acids detected in both 1Â mM CaCl2 and MQ | ||||||
|---|---|---|---|---|---|---|
| Â | % recovery before normalization | % recovery after normalization | CV in % | |||
SPE-CaCl2 | SPE-MQ | SPE-CaCl2 | SPE-MQ | SPE-CaCl2 | SPE-MQ | |
GABA | 27.5 | 79.8 | 35.4 | 106.5 | 5.8 | 6.5 |
His | 79.7 | 82.4 | 102.6 | 109.5 | 9.5 | 7.2 |
Ile | 74.3 | 79.8 | 95.8 | 106.2 | 2.3 | 5.2 |
Leu | 80.7 | 78.8 | 104 | 104.8 | 1.6 | 3.5 |
Lys | 97.1 | 99 | 125.1 | 131.7 | 3.9 | 6.3 |
Phe | 84 | 76.3 | 108.2 | 101.5 | 1.1 | 2.4 |
Pro | 37.8 | 81.7 | 48.7 | 108.6 | 6.7 | 4.5 |
Trp | 53.2 | 49.3 | 68.3 | 65.3 | 25.4 | 19.2 |
Tyr | 77.6 | 82.3 | 99.9 | 109.5 | 2.4 | 4.2 |
Val | 60 | 79.9 | 77.3 | 106.3 | 4.7 | 3.7 |
Nva | 77.7 | 75.3 | – | – | 3.1 | 5.9 |
b. Group 2 of amino acids detected in both 1 mM CaCl2 and MQ | ||||||
|---|---|---|---|---|---|---|
| Â | % recovery before normalization | % recovery after normalization | CV in % | |||
SPE-CaCl2 | SPE-MQ | SPE-CaCl2 | SPE-MQ | SPE-CaCl2 | SPE-MQ | |
Ala | 5.7 | 37 | 7.4 | 49.3 | 29 | 13.4 |
Arg | 1.3 | 24 | 1.6 | 32.5 | 69 | 33.7 |
Asn | 1.6 | 5.4 | 2.1 | 7.3 | 15.9 | 30.2 |
Asp | 1.7 | 8.4 | 2.3 | 11.2 | 26.3 | 37.8 |
Gln | 0.9 | 18.1 | 1.2 | 24.1 | 19 | 7 |
Glu | 1.8 | 21.8 | 2.3 | 29.1 | 12.5 | 9.8 |
Met | 15.6 | 29.7 | 20.2 | 39.1 | 40.4 | 25.2 |
Ser | 4.7 | 12.6 | 6 | 16.2 | 86 | 110.6 |
Thr | 1.7 | 14.1 | 2.3 | 18.8 | 56.9 | 18.2 |