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Fig. 7 | Plant Methods

Fig. 7

From: Development of a split fluorescent protein-based RNA live-cell imaging system to visualize mRNA distribution in plants

Fig. 7

Visualization of mRNA distribution in cells co-expressing MNLS-FPN and PNLS-FPC with FTHSL12 mRNA. Confocal microscopy of N. benthamiana leaves co-expressing A, C MNLS-FPN and PNLS-FPC without target mRNA or B, D with FTHSL12. C and D are the magnified images of the rectangle marked area in A and B, respectively. Note that the punctate fluorescence of FTHSL12 mRNA is detected in the nucleus and along cytoplasmic strands or cell periphery in D. The boundary of the nucleus is indicated by arrows in C and D. Scale Bar = 20 mm in all images

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Plant Methods

ISSN: 1746-4811