Fig. 4
Speckle-like background GFP fluorescence in nucleus of cells with co-expression of MCP-FP and NLS-containing PCP-FP. Confocal microscopy of N. benthamiana leaves co-infiltrated with A, D, G FPN-M and PNLS-FPC; B, E, H M-FPN and PNLS-FPC; or C, F, I MNLS-FPN and PNLS-FPC. D–I Magnified images of A–C. Note that the background GFP fluorescence is detected in both the cytosol and nucleus in A, D, G, whereas the speckle-like background GFP fluorescence is detected in the nucleus in B, C, E, F, H, I. Scale bar: A–C = 50 mm; D–I = 20 mm. The boundary of the nucleus is indicated by white arrows in H and I