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Table 2 The comparison of RNA concentrations mean in various fresh and transportable methods

From: Stability investigation of air-dried olive ribo nucleic acids for metavirome studies

Sample type

Extraction method

Concentration (ng/µL)

CV

A260/280

A260/230

Fresh extractions

TRIzol

455.26 ± 4.54a

0.75

1.72 ± 0.04

0.22 ± 0.02

Modified CTAB 1

330.74 ± 4.38b

0.74

2.03 ± 0.27

0.97 ± 0.13

Ribospin plant RNA extraction kit

33.12 ± 1.2c

0.82

1.40 ± 0.13

0.72 ± 0.19

Modified CTAB 2

14.93 ± 5.3c

0.94

1.39 ± 0.14

0.70 ± 0.17

Air-dried extracted RNA

TRIzol 1

44.87 ± 4.85c

0.65

2.02 ± 0.03

1.21 ± 0.13

TRIzol 2

23.35 ± 3.5c

0.97

1.91 ± 0.04

0.64 ± 0.14

Modified CTAB 1

27.66 ± 3.8c

0.89

2.10 ± 0.04

1.11 ± 0.16

Ribospin plant RNA extraction kit

11.55 ± 1.7c

0.96

1.96 ± 0.05

1.50 ± 0.13

Complementary techniques

Lyophilized leaves

28.04 ± 3.7c

0.46

1.91 ± 0.04

0.64 ± 0.15

Storing in RNAlater

63.46 ± 4.0c

0.28

2.02 ± 0.03

1.20 ± 0.16

  1. Different lowercase letters denote significant differences among RNA concentrations at P ≤ 0.01 by Duncan’s test
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Plant Methods

ISSN: 1746-4811