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Table 1 Histochemical staining patterns of trichomes isolated by various methods

From: An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome

Stain VORTEX STIRRER
RAW PURE RAW PURE
Aniline Blue Distinct signals at the interface between the basal and apical trichome regions (OR) as well as in the branching area
Calcofluor White Homogeneous staining of the whole trichome and occasionally distinct signals at the interface between the basal and apical trichome regions (OR) as well as at the borderlines between stalk and branches
Ruthenium Red Homogeneous red coloration
  Reddish congregations at the trichome surface   Reddish congregations at the trichome surface  
Sudan Black B Black staining of trichome surface and dark congregations inside trichomes
Toluidine Blue O Pinkish-purple staining, either homogeneous or in a speckled pattern Largely transparent or weakly stained after 5 min; pinkish-purple staining after 1 h
Mäule reaction - Homogenous brown staining and occasionally visualization of the OR
  1. OR: Ortmannian Ring