Table 1 Histochemical staining patterns of trichomes isolated by various methods

Aniline Blue

Distinct signals at the interface between the basal and apical trichome regions (OR) as well as in the branching area

Calcofluor White

Homogeneous staining of the whole trichome and occasionally distinct signals at the interface between the basal and apical trichome regions (OR) as well as at the borderlines between stalk and branches

Ruthenium Red

Homogeneous red coloration

Reddish congregations at the trichome surface

Reddish congregations at the trichome surface

Sudan Black B

Black staining of trichome surface and dark congregations inside trichomes

Toluidine Blue O

Pinkish-purple staining, either homogeneous or in a speckled pattern

Largely transparent or weakly stained after 5 min; pinkish-purple staining after 1 h

Mäule reaction

-

Homogenous brown staining and occasionally visualization of the OR