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Table 3 Stability rankings of grapevine candidate reference genes across different sample groups (two tissue types, three developmental stages, infected with or free from GLRaV-3) according to geNorm, NormFinder and BestKeeper

From: Genome-wide screening of novel RT-qPCR reference genes for study of GLRaV-3 infection in wine grapes and refinement of an RNA isolation protocol for grape berries

Rank

Program

geNorm

NormFinder

BestKeeper

Gene

M

Gene

SV

Gene

SD

r

1

CYSP1

0.277983

CYSP1

0.09

CYSP1

0.6

0.951

2

NDUFS81

0.277983

NDUFS81

0.14

NDUFS81

0.53

0.941

3

YLS81

0.401338

YLS81

0.17

YLS81

0.58

0.879

4

Gluc

0.514505

EIF5A2

0.35

Actin2

0.96

0.879

5

EIF5A2

0.535890

Gluc

0.42

Gluc

0.38

0.726

6

GDT1

0.563192

GDT1

0.59

EIF5A2

0.35

0.641

7

EF-hand

0.656230

Actin2

0.70

NAD52

0.57

0.562

8

Actin2

0.872071

EF-hand

0.76

GDT1

0.35

0.51

9

NAD52

3.809479

NAD52

3.78

EF-hand

0.4

0.335

  1. 1The three most stable reference gene candidates are bolded. 2Two conventionally used reference genes were underscored
  2. SV stability value, SD standard deviation, r Pearson coefficient of correlation
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Plant Methods

ISSN: 1746-4811