Fig. 6
From: A high throughput assay of lichenase activity with Congo red dye in plants
Comparative analysis of the fluorescence intensity of CR–lichenan complex in plant preparations after a transient expression of the vectors pLAUMe-GGR, pLAUMe-SynM, pLAUMe-AT30, and pLAUMe-AT65 in Nicotiana benthamiana. For glucuronidase, according to 4-MU and approximated with a logarithmic function of \(\displaystyle f(x) = \ln (x + D) B + C\) type; for clarity, the glucuronidase activity was multiplied by 2000. The calibration curve for lichenase activity was constructed according to purified lichenase and smoothed with a hyperbolic function of \(\displaystyle f(x)=D/(x*A+B)^C\) type. The results were normalized per volume taking into account the used dilutions