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Fig. 2 | Plant Methods

Fig. 2

From: Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells

Fig. 2

Establishment and characterization of mGM-CSF/OsMYBS2RNAi transgenic rice suspension cell lines. A Genotyping of suspension cell lines. PCR-based detection of αAmy3p::mGM-CSF and Ubip::OsMYBS2RNAi chimeric genes was conducted on various calli derived from F3 seeds. The primer sets for mGM-CSF and OsMYBS2RNAi were used to amplify specifically the αAmy3p::mGM-CSF and Ubip::OsMYBS2RNAi chimeric genes, respectively. S2Ri2 and S2Ri6 were OsMYBS2 knockdown lines, G3 and G5 were mGM-CSF transgenic lines, and GS2Ri1 and GS2Ri4 were mGM-CSF/OsMYBS2RNAi lines. B Expression of OsMYBS2, αAmy3, and mGM-CSF in the established rice suspension cell lines. Total RNA was isolated from suspension cells sugar-starved for 2 days and then subjected to qRT-PCR using primers specific for OsMYBS2, αAmy3, and mGM-CSF. Error bars indicate the standard deviation (SD) of triplicate experiments. For OsMYBS2 and αAmy3, gene expression was relative to that of wild-type (WT) suspension cells, where 1 = equivalent. For mGM-CSF, gene expression was relative to that of G3 suspension cells, where 1 = equivalent. * Significantly different from the control cell lines (Student’s t-test: p < 0.05)

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Plant Methods

ISSN: 1746-4811