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Table 3 Flesh color genotype segregation results of the hybrids from the crosses between diploid and tetraploid loquats

From: Genotyping of polyploid plants using quantitative PCR: application in the breeding of white-fleshed triploid loquats (Eriobotrya japonica)

Cross

Ploidy

Flesh color type

Segregation ratio

(R:W)

Expected ratio

(R:W)

Chi-square

P value

Red

White

‘GF’ × B431

2x

4

5

1:1.25

1:3

1.81

0.18

(aa × Aaaa)

3x

5

5

1:1

1:1

0.00

1

 

4x

2

0

1:0

3:1

0.67

0.42

 

Total

11

10

1.1:11

1:1.38

0.92

0.37

B431 × ‘GF’

2x

0

1

0:1

1:3

0.33

0.57

(Aaaa × aa)

3x

20

30

1:1.5

1:1

2.00

0.16

 

4x

0

0

/

/

/

/

 

Total

20

31

1:1.55

1:1.02

2.16

0.16

‘BTZ’ × B432

2x

3

2

1.5:1

1:3

3.27

0.07

(aa × Aaaa)

3x

8

7

1:1.14

1:1

0.07

0.79

 

4x

0

1

0:1

3:1

3.00

0.18

 

Total

11

10

1:1.09

1:1.21

0.43

0.33

B432 × ‘BTZ’

2x

3

4

1:1.33

1:3

1.37

0.25

(Aaaa × aa)

3x

27

19

1.42:1

1:1

1.44

0.23

 

4x

1

1

1:1

3:1

0.67

0.42

 

Total

31

24

1.29:1

1:1.13

2.31

0.24

‘HB1’ × B456

2x

2

1

2:1

1:1

0.33

0.57

(aa × AAaa)

3x

10

0

1:0

5:1

2.00

0.15

 

4x

3

1

3:1

1:1

1.00

0.32

 

Total

15

2

7.5:1

2.28:1

0.91

0.23

B456 × ‘HB1’

2x

0

0

/

/

/

/

(AAaa × aa)

3x

66

14

4.71:1

5:1

0.04

0.84

 

4x

1

1

1:1

1:1

0.00

1

 

5x

3

0

1:0

1:0

0.00

1

 

Total

70

15

4.73:1

3.77:1

0.57

0.84

‘HB1’ × H424

2x

1

0

1:0

1:0

0.00

1

(aa × AAAA)

3x

4

0

1:0

1:0

0.00

1

 

4x

5

0

1:0

1:0

0.00

1

 

Total

10

0

1:0

1:0

0.00

1

H424 × ‘HB1’

2x

0

0

/

/

/

/

(AAAA × aa)

3x

5

0

1:0

1:0

0.00

1

 

4x

0

0

/

/

/

/

 

Total

5

0

1:0

1:0

0.00

1