Fig. 2

Schematic representation of the expected results of an EMSA visualised in an electrophoretic gel. While the first lane presents a band with the size of the segment being tested (WT probe), in the second lane, the introduction of the binding protein and formation of a nucleic acid–protein complex delays the run of this fragment. As for the third lane, the presence of an unlabelled competitor probe leads to the formation of a complex containing this competitor probe and the protein of interest and, consequently, the band that corresponds to the free labelled WT probe appears again. Finally, in the last lane, the replacement of the WT probe by a labelled mutated fragment (mt probe) may cause a similar result to the one of the fist lane, in case the mutation affects the complex formation. Created with BioRender.com