Fig. 7

CRISPR-Cas9 gene editing with soybean hairy roots. a Gene structure and selected sgRNA target of soybean Glyma06g14180. Blue characters indicate sgRNA, Red characters indicate PAM sequence. Underline characters indicate Pst I enzyme site. b, c Electrophoresis and sequencing analysis of target gene editing. The target gene was amplified using gene specific primers and digested with the restriction enzyme (PCR-RE assay). Edited samples produced one large band (arrow indicates), whereas wild-type samples produced two small bands. Sequencing results showed different mutation type. Yellow characters indicate mutation sequence. b Showed results of samples from no herbicide selection rooting medium. c Showed results of samples from rooting medium with herbicide selection. d Editing efficiency of hairy roots from rooting medium with herbicide selection or no selection. Soybean genotype Williams 82 was used here. Values are mean ± SD of three biological replicates, means with different letters denote a significance difference while similar letters denote no significance (Student’s t-test, P < 0.05). *Indicates significant difference at 0.05 level by student’s t-test