Fig. 1
From: Application of CRISPR/Cas9 technology in wild apple (Malus sieverii) for paired sites gene editing
The structure of the MsPDS gene, target site selection, and schematic diagram of the Cas9/sgRNA vector. A The structure of the MsPDS gene. The black rectangles represent the different exons. B The target sites of different exons. The yellow number is the position of the exon. The red text indicates the PAM. C The vector ID of paired-sgRNA/Cas9 binary vectors for both the CRISPR/Cas9 and pTG-CRISPR/Cas9 systems. D Schematic depicting the paired sgRNA expression cassettes of both the CRISPR/Cas9 and pTG-CRISPR/Cas9 systems. Diamonds of different colours represent sgRNA, rectangles represent the sgRNA scaffold, and purple rounded rectangles represent tRNA