Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter

Table 1 Composition of different media tested for induction of callus in Stipagrostis pennata

Name of medium	Base	BAP 0.4 mg·L^–1	2,4-D 3 mg·L^–1	Myo- Inositol 100 mg·L⁻¹	Casein hydrolysate100 mg·L⁻¹	Ascorbic acid 100 mg·L^–1	CuSO₄ 0.5 mg·L^–1	Sucrose g·L^–1	Maltose 10 g·L^–1	l-Glutamine 500 mg·L^–1	Gelrite 3 g·L^–1
MS-T	MS	√	√	√	−	−	√	30	−	−	√
MS-Pw	MS	−	√	√	−	−	√	30	−	−	√
MS-Mod	MS	−	√	√	√	√	√	20	√	√	√
MS-FS	MS	−	√	√	√	√	√	20	√	√	√

MS-T (MS from stock solutions used in Teheran; autoclaved), MS-Pw (MS powder from Duchefa; autoclaved), MS-Mod (MS powder from Duchefa; autoclaved), MS-FS (MS powder from Duchefa; filter-sterilized)

ISSN: 1746-4811