Skip to main content
Fig. 1 | Plant Methods

Fig. 1

From: Protocol development for somatic embryogenesis, SSR markers and genetic modification of Stipagrostis pennata (Trin.) De Winter

Fig. 1

Somatic embryogenesis of Stipagrostis pennata: a mature seeds, b zygotic embryo, c induction of embryogenic callus, d induction of shoot on MS medium supplemented with zeatin riboside, e elongation of shoot on MS hormone free medium f rooted in vitro plants on MS hormone free medium, g plants adapted in greenhouse, h spike with the flower; arrow is showing anthers and stigma, i section of preglobular somatic embryo stage stained with toluidine blue, j section of globular embryo, k section of emryoids an advanced stage of somatic embryo l control embryogenic callus for gus gene expression, m gus gene expression in embryogenic callus

Back to article page

Plant Methods

ISSN: 1746-4811