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Fig. 2 | Plant Methods

Fig. 2

From: Use of the rhizobial type III effector gene nopP to improve Agrobacterium rhizogenes-mediated transformation of Lotus japonicus

Fig. 2

Formation of hairy roots on L. japonicus seedlings induced by different A. rhizogenes strains carrying pISV-DsRed1. The binary vector pISV-DsRed1 was introduced into the A. rhizogenes strains LBA1334, K599 and LBA9402. Each strain was inoculated on 50 plants. Plants were analyzed at 28 dpi. Data indicate means ± SE. Different letters above columns indicate statistically significant differences (Duncan’s Multiple Range test, P < 0.05). a Total number of formed hairy roots per plant (n = 50). b Microscopic analysis of formed hairy roots under bright field conditions (top) and for red fluorescence (RF) emission (bottom). Bar = 500 μm. c Transformation efficiency as determined by the percentage of red fluorescent hairy roots per plant. Data were obtained from plants showing at least one red fluorescent root (n = 8 for LBA1334; n = 20 for K599; n = 23 for LBA9402)

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Plant Methods

ISSN: 1746-4811