Table 2 Growth conditions for conidia production in Pyrenophora sp. Columns differentiate growth phases and row describe parameters. All listed conditions were significantly associated with increased conidia production and purity
From: An optimized sporulation method for the wheat fungal pathogen Pyrenophora tritici-repentis
Parameters | Vegetative | Conidiophore | Conidia | Harvest |
|---|---|---|---|---|
Duration | 5–7 days | 24 h | 18 h | – |
Light Source | White | N-UV | Darkness | – |
Light Cycle | 12 h Light/Dark | Continuous | Continuous | – |
Temperature | 22–24 °C | 22–24 °C | 15 °C | – |
Plate Lid | Sealed | Removed | Loose | – |
Media | V8-PDA | V8-PDA | – | 0.02% Tween 20 |
Plug Location | Total Mycelia | – | – | – |
Wounding Method | 200 µl Tip End | – | – | – |
Extraction | – | – | – | Small brush Excess agar removed |