Table 1 Method validation
From: A stable isotope dilution method for a highly accurate analysis of karrikins
Compound | Calibration curve | Determinated spiked KARs content [pmol] | Method precision [RSD%] | Method accuracy [%bias] | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
1 | 5 | 10 | 1 | 5 | 10 | 1 | 5 | 10 | ||
KAR1 | Cal 1 | 1.27 ± 0.03 | 5.60 ± 0.05 | 11.09 ± 0.49 | 2.4 | 1.0 | 4.5 | 27.1 | 12.0 | 10.9 |
Cal 2 | 1.58 ± 0.04 | 6.89 ± 0.07 | 13.57 ± 0.60 | 2.4 | 1.0 | 4.4 | 58.0 | 37.7 | 35.7 | |
Cal 3 | 1.08 ± 0.03 | 5.39 ± 0.06 | 11.29 ± 0.54 | 2.6 | 1.0 | 4.8 | 8.2 | 7.7 | 12.9 | |
KAR2 | Cal 1 | 2.79 ± 0.12 | 10.50 ± 0.28 | 20.72 ± 0.74 | 4.2 | 2.6 | 3.6 | 178.8 | 110.1 | 107.2 |
Cal 2 | 1.72 ± 0.08 | 7.39 ± 0.21 | 15.61 ± 0.61 | 4.6 | 2.9 | 3.9 | 71.5 | 47.8 | 56.1 | |
Cal 3 | 1.06 ± 0.05 | 4.47 ± 0.13 | 9.34 ± 0.36 | 4.5 | 2.9 | 3.9 | 6.0 | − 10.6 | − 6.6 | |