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Table 1 Method validation

From: A stable isotope dilution method for a highly accurate analysis of karrikins

Compound Calibration curve Determinated spiked KARs content [pmol] Method precision [RSD%] Method accuracy [%bias]
1 5 10 1 5 10 1 5 10
KAR1 Cal 1 1.27 ± 0.03 5.60 ± 0.05 11.09 ± 0.49 2.4 1.0 4.5 27.1 12.0 10.9
Cal 2 1.58 ± 0.04 6.89 ± 0.07 13.57 ± 0.60 2.4 1.0 4.4 58.0 37.7 35.7
Cal 3 1.08 ± 0.03 5.39 ± 0.06 11.29 ± 0.54 2.6 1.0 4.8 8.2 7.7 12.9
KAR2 Cal 1 2.79 ± 0.12 10.50 ± 0.28 20.72 ± 0.74 4.2 2.6 3.6 178.8 110.1 107.2
Cal 2 1.72 ± 0.08 7.39 ± 0.21 15.61 ± 0.61 4.6 2.9 3.9 71.5 47.8 56.1
Cal 3 1.06 ± 0.05 4.47 ± 0.13 9.34 ± 0.36 4.5 2.9 3.9 6.0 − 10.6 − 6.6
  1. Analytical precision (RSD%) and accuracy (%bias) of whole procedure shown for different amounts of karrikins (1, 5 and 10 pmol). The extract of 10 mg (FW) Arabidopsis sample was spiked from 1 to 10 pmol of authentic KAR standards, purified by SPE and analysed by UHPLC-MS/MS. Concentrations of KARs were quantified using the standard isotope dilution method combined with calibration curves without (Cal 1) and with (Cal 2 and 3) plant matrix. Values are means ± SD (n = 3)