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Fig. 4 | Plant Methods

Fig. 4

From: A stable isotope dilution method for a highly accurate analysis of karrikins

Fig. 4

Optimized protocol for KAR determination in plant tissues. a Scheme of sample microextraction and purification using one-step solid phase extraction with Oasis HLB columns (30 mg/1 ml). b Representative multiple-reaction monitoring chromatograms of KAR1 and KAR2 with appropriate internal standard [2H3]KAR1 containing 1 pmol of each derivative per injection separated by optimized UHPLC–MS/MS method. FA – formic acid, MeOH – methanol, MRM – multi reaction monitoring

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