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Fig. 9 | Plant Methods

Fig. 9

From: Evaluation of genetic stability in olive callus-induced and meristem-induced shoots using flow cytometry and amplified fragment length polymorphism techniques

Fig. 9

Schematic flow diagram of olive (O. europaea L.) ˈAminˈ, ˈArbequinaˈ and ˈMeshkatˈ micropropagation: well-grown single node cuttings were surface-sterilized and established in modified OM (OM supplemented with 4 mg/L zeatin and 0.5 mg/L 2iP). During proliferation stage, regarding the media with unintentionally callus formation (high zeatin media, mannitol enriched media and lower light intensity condition), CIS and MIS were proliferated separately. In vitro plantlets were subcultured for at least 126 days. Then leaf materials were tested for genetic stability assessment by AFLP and FCM

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Plant Methods

ISSN: 1746-4811