Fig. 6
From: Optimization of protoplast regeneration in the model plant Arabidopsis thaliana
Schematic overview of the Arabidopsis protoplast regeneration process. Time required for each step is indicated in parentheses. Freshly isolated protoplasts are immobilized in a thin alginate hydrogel. Protoplasts start dividing upon incubation in PIM and produce callus by incubation in CIM. De novo shoot regeneration is facilitated by incubation on SIM for 3 weeks. Inflorescence stem explants are used for efficient de novo root regeneration. Plantlets are first grown on RM for 3 weeks to induce root regeneration and then transferred to soil to complete their life cycle and produce progenies. The whole process can be completed within 15 weeks