Fig. 4
From: Cut and paste: temperature-enhanced cotyledon micrografting for Arabidopsis thaliana seedlings
Successful replacement of cotyledons does not result in deficiency in biomass production. The impact of cot-grafting on plant development was investigated by evaluating biomass production at two different developmental stages after grafting. a Illustration of the biomass evaluation workflow. b–d Fresh weight measurements of whole seedlings 15 days after sowing (DAS; b). Above ground biomass of single plants 35 DAS (c), and number of rosette leaves 35 DAS (d). Cot-grafted plants (grafted cotyledon—GC) were compared to three controls: intact plants with two cotyledons (2C), plants without cotyledons (0C) and plants with one cotyledon removed (1C). N = 15; different letters denote statistically significant differences as assessed by 1-way ANOVA followed by a Tukey HSD test (p < .05). SUC, sucrose; ATS, A. thaliana solution medium