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Fig. 3 | Plant Methods

Fig. 3

From: Comprehensive comparison and applications of different sections in investigating the microstructure and histochemistry of cereal kernels

Fig. 3

An improved method for preparing the whole section of cereal mature kernels aided with ultramicrotome and its applications in observing starch morphology (cited from Zhao et al. [35]). a–j The preparing processes of section. e, f The polished surface of chalky or floury kernel is treated with nail polish (e), and then be polished again after nail polish solidification (f). This treatment of nail polish is omitted if kernels are translucent or vitreous. k The morphology and distribution of heterogeneous starch granules in different regions of high-amylose maize kernels with floury endosperm. The section is stained with iodine solution, and the region 1, 2, 3 and 4 in whole section are enlarged. Scale bar = 1 mm for whole section and 10 μm for regional magnification

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