Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

Table 3 Rosette area (mm²) and RWC (%) of Arabidopsis thaliana wild type (WT), proCaMV35S > GR > HvCKX2 (CKX) and proCaMV35S > GR > ipt (IPT) plants after half of the SRS had been activated with DEX and cut from the main plant the following day

	Area 21 DAS		Area 24 DAS		Area 26 DAS		Area 30 DAS		Final RWC
WT
Watered	183 ± 6 a	10%	372 ± 19 a	19%	612 ± 22 a	28%	1121 ± 40 a	33%	92 ± 2 a
Drought	165 ± 6 a	10%	300 ± 17 b	19%	441 ± 20 b	28%	745 ± 34 b	33%	83 ± 3 b
Watered-Cut	182 ± 10 a	38%	369 ± 22 a	46%	558 ± 36 a	55%	1003 ± 63 a	63%	92 ± 2 a
Dorught-Cut	112 ± 7 b	38%	197 ± 19 c	46%	253 ± 20 c	55%	368 ± 33 c	63%	74 ± 2 c
CKX
Watered	181 ± 9 a	7%	301 ± 21 a	7%	442 ± 26 a	9%	815 ± 48 a	17%	91 ± 1 a
Drought	168 ± 8 ab	7%	279 ± 21 a	7%	402 ± 22 a	9%	678 ± 34 b	17%	85 ± 2 b
Watered-Cut	151 ± 7 ab	10%	280 ± 18 a	28%	406 ± 26 a	31%	723 ± 49 ab	39%	90 ± 1 a
Dorught-Cut	137 ± 5 b	10%	202 ± 13 b	28%	282 ± 13 b	31%	443 ± 20 c	39%	76 ± 2 c
IPT
Watered	178 ± 10 a	8%	375 ± 24 a	13%	567 ± 32 a	20%	1025 ± 50 a	26%	88 ± 1 a
Drought	165 ± 11 a	8%	327 ± 26 a	13%	454 ± 26 b	20%	756 ± 42 b	26%	78 ± 2 b
Watered-Cut	177 ± 21 a	22%	362 ± 40 a	32%	527 ± 54 ab	37%	938 ± 71 a	56%	86 ± 1 a
Dorught-Cut	138 ± 7 b	22%	247 ± 17 b	32%	331 ± 18 c	37%	410 ± 24 c	56%	72 ± 2 c

Different letters mean statistically significant differences (within each genotype) for p < 0.05, according to Student’s t test (n ≥ 10)
The percentual number indicates the decrease in rosette area (in %) between watered and drought plants and between watered-cut and drought-cut plants. Water was withdrawn from drought treatment starting 10 DAS, plants were watered one more time 16 days after sowing, and the watered side of the SRS was cut 17 DAS. Afterwards, the remaining half of the root system was kept under either watered or drought conditions

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