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Table 3 Rosette area (mm2) and RWC (%) of Arabidopsis thaliana wild type (WT), proCaMV35S > GR > HvCKX2 (CKX) and proCaMV35S > GR > ipt (IPT) plants after half of the SRS had been activated with DEX and cut from the main plant the following day

From: Split-root systems: detailed methodology, alternative applications, and implications at leaf proteome level

  Area 21 DAS Area 24 DAS Area 26 DAS Area 30 DAS Final RWC
WT
 Watered 183 ± 6 a 10% 372 ± 19 a 19% 612 ± 22 a 28% 1121 ± 40 a 33% 92 ± 2 a
 Drought 165 ± 6 a 300 ± 17 b 441 ± 20 b 745 ± 34 b 83 ± 3 b
 Watered-Cut 182 ± 10 a 38% 369 ± 22 a 46% 558 ± 36 a 55% 1003 ± 63 a 63% 92 ± 2 a
 Dorught-Cut 112 ± 7 b 197 ± 19 c 253 ± 20 c 368 ± 33 c 74 ± 2 c
CKX
 Watered 181 ± 9 a 7% 301 ± 21 a 7% 442 ± 26 a 9% 815 ± 48 a 17% 91 ± 1 a
 Drought 168 ± 8 ab 279 ± 21 a 402 ± 22 a 678 ± 34 b 85 ± 2 b
 Watered-Cut 151 ± 7 ab 10% 280 ± 18 a 28% 406 ± 26 a 31% 723 ± 49 ab 39% 90 ± 1 a
 Dorught-Cut 137 ± 5 b 202 ± 13 b 282 ± 13 b 443 ± 20 c 76 ± 2 c
IPT
 Watered 178 ± 10 a 8% 375 ± 24 a 13% 567 ± 32 a 20% 1025 ± 50 a 26% 88 ± 1 a
 Drought 165 ± 11 a 327 ± 26 a 454 ± 26 b 756 ± 42 b 78 ± 2 b
 Watered-Cut 177 ± 21 a 22% 362 ± 40 a 32% 527 ± 54 ab 37% 938 ± 71 a 56% 86 ± 1 a
 Dorught-Cut 138 ± 7 b 247 ± 17 b 331 ± 18 c 410 ± 24 c 72 ± 2 c
  1. Different letters mean statistically significant differences (within each genotype) for p < 0.05, according to Student’s t test (n ≥ 10)
  2. The percentual number indicates the decrease in rosette area (in %) between watered and drought plants and between watered-cut and drought-cut plants. Water was withdrawn from drought treatment starting 10 DAS, plants were watered one more time 16 days after sowing, and the watered side of the SRS was cut 17 DAS. Afterwards, the remaining half of the root system was kept under either watered or drought conditions
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