A new method for functional analysis of plastid EMBRYO-DEFECTIVE PPR genes by efficiently constructing cosuppression lines in Arabidopsis

Table 1 The cosuppression phenotype and frequency of transgenic plants expressing various constructs in WT

Plamid construct^a	Length of encoding protein (aa)	Number of PPR motifs	Phenotype of cosuppression	Number of total T₁ transformants	Number of T₁ transformants with visible chlorosis	Frequency of cosuppression^b	Severity of chlorosis in cosuppression lines
SOT5	978	11	Yellow inflorescence and cauline leaves	35	20	57%	Very mild
SOT5-m1	712	6	Albino young leaves at seedling stage	30	24	80%	Strong
SOT5-m2	1006	10	Partial albino leaves at seedling stage	42	20	48%	Mild
EMB2654	822	18	ND	ND	ND	ND	ND
EMB2654-11M	550	11	Chlorosis leaves at seedling stage	47	47	100%	Mild
EMB976	1038	22	ND	ND	ND	ND	ND
EMB976-7M	443	7	WT-like	13	0	0%	ND
EMB976-14M	747	14	Yellow young leaves at seedling stage	8	4	50%	Mild

^aThe pGWB2 plasmid containing the mutated or truncated CDS indicated by the name
^bCosuppressed T₁ tranfromants/total T₁ transformants
ND not determined

ISSN: 1746-4811