Skip to main content
Fig. 3 | Plant Methods

Fig. 3

From: A new method for functional analysis of plastid EMBRYO-DEFECTIVE PPR genes by efficiently constructing cosuppression lines in Arabidopsis

Fig. 3

No chlorosis leaf phenotypes appeared when the mutated SOT5 CDS were overexpressed in rdr6-11 background. a Phenotype of transgenic plants overexpressing SOT5-m1 and SOT5-m2 in rdr6-11 (bar = 1.5 cm). b RT-PCR analysis of mRNA levels of endogenous and total SOT5, and two plastid genes in transgenic lines. c RT-qPCR analysis of the endogenous SOT5 transcript level in transgenic lines. d RT-qPCR analysis of the total SOT5 expression level in transgenic lines. e Splicing efficiency of plastid rpl2 and atpF (as a control) in transgenic lines. For RT-qPCR, the values are means of three technique replicates (bars indicate SD). Asterisks indicate significant differences between wild type (WT) and transgenic plants (Student’s t test, *, P < 0.05 and **, P < 0.01). Two to three T1 transformants were analyzed for RT-PCR and RT-qPCR

Back to article page

Plant Methods

ISSN: 1746-4811