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Fig. 3 | Plant Methods

Fig. 3

From: Efficient isolation of protoplasts from rice calli with pause points and its application in transient gene expression and genome editing assays

Fig. 3

Protoplast viability and size over time. a Viability of protoplasts was measured by counting protoplasts unstained by Evans Blue dye on a hemocytometer. Total live protoplasts were calculated by first determining protoplast density, then multiplying by the total volume of protoplasts from the isolation. Counts were performed in triplicate. The means are plotted and error bars indicate standard deviation. b A random sampling of 50 protoplasts was measured for diameter at 0 and 7 days in MMG. Each measurement was plotted individually, and the means were indicated by a horizontal line

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Plant Methods

ISSN: 1746-4811