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Table 2 Expression stability rankings of 10 candidate RGs using the ΔCt method, geNorm, NormFinder and BestKeeper

From: Evaluation of duplicated reference genes for quantitative real-time PCR analysis in genome unknown hexaploid oat (Avena sativa L.)

Ranking numbers 1 2 3 4 5 6 7 8 9 10
All samples ΔCt 18S (0.95) EIF4A (1.67) TBC (1.71) UBQ10 (1.74) UBC21 (1.82) HNR (1.85) EF1A (1.94) EP (2.02) PP2A (2.05) GAPDH1 (2.06)
geNorm HNR (0.68) EIF4A (0.70) EF1A (0.71) PP2A (0.78) UBC21 (0.78) TBC (0.83) EP (0.92) GAPDH1 (0.93) UBQ10 (1.09) 18S (1.33)
NormFinder EIF4A (0.15) HNR (0.21) EF1A (0.24) UBC21 (0.34) PP2A (0.35) TBC (0.38) GAPDH1 (0.49) EP (0.51) UBQ10 (0.62) 18S (0.85)
BestKeeper 18S (0.78) EIF4A (1.42) UBQ10 (1.43) TBC (1.51) HNR (1.68) GAPDH1 (1.68) UBC21 (1.69) EF1A (1.74) EP (1.80) PP2A (1.80)
Seedlings ΔCt UBC21 (0.34) GAPDH1 (0.50) EP (0.51) HNR (0.56) EF1A (0.57) UBQ10 (0.60) EIF4A (0.60) PP2A (0.73) 18S (0.80) TBC (0.91)
geNorm EIF4A (0.39) HNR (0.42) EP (0.44) UBQ10 (0.45) PP2A (0.45) EF1A (0.50) UBC21 (0.51) TBC (0.65) 18S (0.76) GAPDH1 (0.85)
NormFinder HNR (0.13) PP2A (0.14) UBC21 (0.18) EIF4A (0.21) EP (0.29) UBQ10 (0.35) EF1A (0.35) TBC (0.40) GAPDH1 (0.42) 18S (0.63)
BestKeeper UBC21 (0.25) EP (0.38) GAPDH1 (0.44) EF1A (0.45) HNR (0.48) EIF4A (0.55) UBQ10 (0.57) PP2A (0.65) 18S (0.66) TBC (0.85)
Seeds ΔCt 18S (1.32) TBC (2.12) EIF4A (2.16) EP (2.23) HNR (2.26) UBC21 (2.34) PP2A (2.37) EF1A (2.39) UBQ10 (2.46) GAPDH1 (2.83)
geNorm EF1A (0.56) EP (0.56) HNR (0.58) PP2A (0.61) EIF4A (0.61) UBC21 (0.71) TBC (0.83) UBQ10 (0.86) GAPDH1 (0.90) 18S (1.18)
NormFinder EF1A (0.11) EP (0.12) HNR (0.16) PP2A (0.19) EIF4A (0.22) UBC21 (0.33) TBC (0.46) UBQ10 (0.48) GAPDH1 (0.55) 18S (0.76)
BestKeeper 18S (1.19) EIF4A (1.99) TBC (2.00) EP(2.03) HNR (2.06) UBQ10 (2.13) PP2A (2.18) UBC21 (2.20) EF1A (2.24) GAPDH1 (2.45)
Endosperms ΔCt 18S (0.54) EIF4A (0.87) TBC (0.92) UBC21 (1.08) EP (1.13) HNR (1.13) UBQ10 (1.34) PP2A (1.36) EF1A (1.52) GAPDH1 (1.63)
geNorm HNR (0.51) PP2A (0.52) UBC21 (0.55) EIF4A (0.57) EP (0.63) EF1A (0.64) UBQ10 (0.68) GAPDH1 (0.70) TBC (0.72) 18S (0.98)
NormFinder EIF4A (0.06) HNR (0.06) UBQ10 (0.14) EP (0.14) PP2A (0.16) EF1A (0.24) UBC21 (0.24) TBC (0.40) 18S (0.48) GAPDH1 (0.57)
BestKeeper 18S (0.41) EIF4A (0.65) TBC (0.79) UBC21 (0.89) HNR (0.90) EP (1.03) PP2A (1.05) EF1A (1.18) UBQ10 (1.20) GAPDH1 (1.24)
  1. Values in brackets are STDEV for ΔCt, M values for geNorm, SVs (stability values) for NormFinder and CV ± SD values for BestKeeper (CV the coefficient of variance, SD standard deviation)