Table 2 Expression stability rankings of 10 candidate RGs using the ΔCt method, geNorm, NormFinder and BestKeeper
Ranking numbers | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |
|---|---|---|---|---|---|---|---|---|---|---|---|
All samples | ΔCt | 18S (0.95) | EIF4A (1.67) | TBC (1.71) | UBQ10 (1.74) | UBC21 (1.82) | HNR (1.85) | EF1A (1.94) | EP (2.02) | PP2A (2.05) | GAPDH1 (2.06) |
geNorm | HNR (0.68) | EIF4A (0.70) | EF1A (0.71) | PP2A (0.78) | UBC21 (0.78) | TBC (0.83) | EP (0.92) | GAPDH1 (0.93) | UBQ10 (1.09) | 18S (1.33) | |
NormFinder | EIF4A (0.15) | HNR (0.21) | EF1A (0.24) | UBC21 (0.34) | PP2A (0.35) | TBC (0.38) | GAPDH1 (0.49) | EP (0.51) | UBQ10 (0.62) | 18S (0.85) | |
BestKeeper | 18S (0.78) | EIF4A (1.42) | UBQ10 (1.43) | TBC (1.51) | HNR (1.68) | GAPDH1 (1.68) | UBC21 (1.69) | EF1A (1.74) | EP (1.80) | PP2A (1.80) | |
Seedlings | ΔCt | UBC21 (0.34) | GAPDH1 (0.50) | EP (0.51) | HNR (0.56) | EF1A (0.57) | UBQ10 (0.60) | EIF4A (0.60) | PP2A (0.73) | 18S (0.80) | TBC (0.91) |
geNorm | EIF4A (0.39) | HNR (0.42) | EP (0.44) | UBQ10 (0.45) | PP2A (0.45) | EF1A (0.50) | UBC21 (0.51) | TBC (0.65) | 18S (0.76) | GAPDH1 (0.85) | |
NormFinder | HNR (0.13) | PP2A (0.14) | UBC21 (0.18) | EIF4A (0.21) | EP (0.29) | UBQ10 (0.35) | EF1A (0.35) | TBC (0.40) | GAPDH1 (0.42) | 18S (0.63) | |
BestKeeper | UBC21 (0.25) | EP (0.38) | GAPDH1 (0.44) | EF1A (0.45) | HNR (0.48) | EIF4A (0.55) | UBQ10 (0.57) | PP2A (0.65) | 18S (0.66) | TBC (0.85) | |
Seeds | ΔCt | 18S (1.32) | TBC (2.12) | EIF4A (2.16) | EP (2.23) | HNR (2.26) | UBC21 (2.34) | PP2A (2.37) | EF1A (2.39) | UBQ10 (2.46) | GAPDH1 (2.83) |
geNorm | EF1A (0.56) | EP (0.56) | HNR (0.58) | PP2A (0.61) | EIF4A (0.61) | UBC21 (0.71) | TBC (0.83) | UBQ10 (0.86) | GAPDH1 (0.90) | 18S (1.18) | |
NormFinder | EF1A (0.11) | EP (0.12) | HNR (0.16) | PP2A (0.19) | EIF4A (0.22) | UBC21 (0.33) | TBC (0.46) | UBQ10 (0.48) | GAPDH1 (0.55) | 18S (0.76) | |
BestKeeper | 18S (1.19) | EIF4A (1.99) | TBC (2.00) | EP(2.03) | HNR (2.06) | UBQ10 (2.13) | PP2A (2.18) | UBC21 (2.20) | EF1A (2.24) | GAPDH1 (2.45) | |
Endosperms | ΔCt | 18S (0.54) | EIF4A (0.87) | TBC (0.92) | UBC21 (1.08) | EP (1.13) | HNR (1.13) | UBQ10 (1.34) | PP2A (1.36) | EF1A (1.52) | GAPDH1 (1.63) |
geNorm | HNR (0.51) | PP2A (0.52) | UBC21 (0.55) | EIF4A (0.57) | EP (0.63) | EF1A (0.64) | UBQ10 (0.68) | GAPDH1 (0.70) | TBC (0.72) | 18S (0.98) | |
NormFinder | EIF4A (0.06) | HNR (0.06) | UBQ10 (0.14) | EP (0.14) | PP2A (0.16) | EF1A (0.24) | UBC21 (0.24) | TBC (0.40) | 18S (0.48) | GAPDH1 (0.57) | |
BestKeeper | 18S (0.41) | EIF4A (0.65) | TBC (0.79) | UBC21 (0.89) | HNR (0.90) | EP (1.03) | PP2A (1.05) | EF1A (1.18) | UBQ10 (1.20) | GAPDH1 (1.24) | |