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Fig. 2 | Plant Methods

Fig. 2

From: A fluorescence-based high-throughput screening method for cytokinin translocation mutants

Fig. 2

ARR5::eGFP serves as a cytokinin reporter. a Morphological phenotypes of ARR5::eGFP Col-4 (left) and ARR5::eGFP abcg14 (right). Seeds were sowed on the square plates (10 cm * 10 cm) and grown vertically for 6 days. Scale bar, 1 cm. b Fluorescence signal in the roots of transgenic plants of ARR5::eGFP in Col-4 (left) and atabcg14 mutants (right) background observed under the LIS. c, d Fluorescence signal in the true leaves of ARR5::eGFP Col-4 (left) and ARR5::eGFP abcg14 (right) observed under the fluorescence microscope. Scale bar, 500 μm. e, f Fluorescence signal in the roots of transgenic plants of ARR5::eGFP Col-4 (left) and ARR5::eGFP abcg14 (right) observed under the fluorescence microscope. Scale bar, 500 μm

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