Fig. 2
From: An improved pollen number counting method using a cell counter and mesh columns
Flowchart of the pollen grain counting technique. Outline of the protocol and the corresponding procedure steps. a Male strobilus is crushed gently in a 1.5-mL tube and suspended by DW (250 μL × 2). b–d Pollen suspension is transferred to 100-μm mesh column and centrifuged to remove bigger debris. e, f The flowthrough is transferred to a 20-μm mesh column and centrifuged to remove small debris. g Pollen-containing particles are mixed with water again (500 μL × 2). Part of pollen suspension (200 μL of 1 ml) is mixed with 10 mL of CASYton. h Particle numbers are counted by a cell counter