Fig. 8

Analysis of Populus trichocarpa stem cell organization by tessellation-based automatic segmentation of an LM image. a Illustration of a centroid coordinate read-out in Populus trichocarpa stem cells produced by ImageJ. Green arrows indicate the direction of the x and y axes, and the number indicates the centroid coordinate of the red point. b Graphical user interface of SR-Tesseler software, including the console, control window, and viewer panel. c Merged image of Populus trichocarpa stem cell particles identified by ImageJ and centroid (red points) displayed in the SR-Tesseler viewer window. d–f Segmentation and quantification of experimental data according to the centroid in c. Polygon creation and establishment of Voronoï diagrams based on the local density (d), mean distance (e), and area (f). Empty polygons are shown above, and filled polygons are shown below. All polygons were merged with the particles identified from Populus trichocarpa stem cells. The polygons were pseudocolor-coded with respect to the segmentation results. g The connection pattern of clusters calculated from established objects. All bars in this figure represent 10 μm