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Fig. 5 | Plant Methods

Fig. 5

From: Anthocyanin, a novel and user-friendly reporter for convenient, non-destructive, low cost, directly visual selection of transgenic hairy roots in the study of rhizobia-legume symbiosis

Fig. 5

CRISPR/Cas9-mediated knockout of Rfg1 in the soybean PI377578 (Nod-) background inoculated with USDA193. a Mature nodules formed on overexpression-AtMyb75 hairy roots. Close-up of section a marked in the white box was shown in section b. c A sequencing identification from CRISPR/Cas9-mediated knockout of Rfg1 in the soybean PI 377578 (Nod-) background. An example of sequencing analysis of the DNA from wild type nodule (WT) and targeted site and PAM site (AGG) are shown within the box. Transgenic nodules revealed that two mutant alleles with a 52-bp (mutant 1#) and 152-bp (mutant 2#) deletion were caused, respectively. The deleted site is shown by a black arrow. Bars = 1 cm

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Plant Methods

ISSN: 1746-4811